Cisplatin dna cross-linking
WebJul 23, 1996 · Abstract cis-Diamminedichloroplatinum (II) (cisplatin) is a widely used anticancer drug that binds to and crosslinks DNA. The major DNA adduct of the drug results from coordination of two adjacent guanine bases to platinum to form the intrastrand crosslink cis- [Pt (NH3)2 [d (GpG)-N7 (1), -N7 (2)]] (cis-Pt-GG). WebDec 15, 1995 · The widely used antitumor drug cis -diamminedichloroplatinum (II) (cisplatin or cis -DDP) reacts with DNA, cross-linking two purine residues through the N7 …
Cisplatin dna cross-linking
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WebJun 24, 2011 · For example, cisplatin, the most widely used crosslinking drug, causes damage that is estimated to comprise of 90% intrastrand crosslinks (predominantly crosslinks between adjacent purine... WebPhoto-cross-linking is thus an effective tool for investigating the interaction of cisplatin-modified DNA with damage-recognition proteins under heterogeneous conditions such …
WebThe platinum 1,3-d(GXG) intrastrand cross-link is one of the adducts formed in the reaction of the antitumor drug cisplatin with DNA, and in fact the major adduct found in cells treated with the cisplatin analogue carboplatin. ... cross-link and other Pt-DNA cross-links sheds new light on the observed differences in protein recognition of these ... WebTumour cells isolated from 50 ovarian cancer patients were treated ex vivo with 100 μ M cisplatin for 1 h and crosslink formation and repair (unhooking) measured. ... in cellular mechanisms such as drug transport or detoxification. In contrast, the percentage repair (unhooking) of DNA interstrand crosslinks was much greater in the group of ...
WebJun 20, 2024 · It has long been established that the mechanism of cis- DDP involves DNA intrastrand cross-link formation ( 6, 7 ), altering the molecular conformation of the double helix leading to apoptosis. Webwww.ncbi.nlm.nih.gov
WebAs we have seen from studies into the mode of action of cisplatin, covalent intrastrand cross-links can also be formed by chemical agents, such as cisplatin. A similar mechanism is believed to repair damage done to cancer cells by cisplatin, as we will see when we discuss drug resistance.
WebCisplatin induces DNA damage through inter-strand or intra-strand cross-linking of DNA . Its dose-limiting toxicity is nephrotoxicity, peripheral nerve toxicity, and toxicity to the cochlea (the inner ear toxicity) . Paclitaxel is a microtubule poison that … can hiatal hernia cause nausea and vomitingWebROS can induce single or double-strand breakage, nucleotide alterations, deoxyribose changes, and DNA cross-linking in DNA. If oxidative DNA damage is not repaired before DNA replication, it could lead to cell death, DNA mutation, replication mistakes, and genomic instability (Cooke et al., 2003; Schupp et al., 2016). fit for the kingWebFeb 1, 2002 · To overcome this obstacle, researchers have focussed their attention on cisplatin (cis-DDP; cis-platinum (II)diamminedichloride), a cross-linking agent shown to … fit for the kingdomfit for the king bookWebNov 15, 1987 · Two sublines were derived from a human bladder carcinoma continuous cell line (RT112-P), one by exposure to fractionated X-irradiation (RT112-DXR8) and the other by continuous exposure to cisplatin (RT112-CP). RT112-DXR8 cells were 1.6- to 2-fold more sensitive to cisplatin and 2 analogues, carbopla … can hiatal hernia cause sobWebCisplatin is one of the most widely used agents in cancer chemotherapy. Its mode of action is cross-linking of the DNA, which can kill cells. But which part of the genome is more … can hiatal hernia cause rapid heartbeatWebSep 28, 2012 · DNA cross-linking agents such as the nitrogen mustards (melphalan, chlorambucil, cyclophosphamide, ifosfamide), platinum drugs (cisplatin, carboplatin, oxaliplatin), chloroethylnitrosoureas (carmustine, lomustine), the alkylalkane sulphonate busulphan and the natural product mitomycin C are widely used drugs as both single … can hiatal hernia cause sleep problems