E2a antibody

Author: rmcn

August undefined, 2024

WebWestern Blot: TCF3/E2A Antibody [NBP2-20585] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:2000. Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585] - Jurkat whole cell extract A. 35 ug Jurkat whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of TCF3 protein by 4 ug TCF3 ... WebTCF-3/E2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44061) at 10 µg/mL …

Feasibility of Generating Adeno-Associated Virus Packaging Cell …

WebFeb 2, 1999 · The anti-human E2A antibody G98–271, the antibody 382.6 (specific for E12, HEB, and E2-2), and goat anti-mouse IgG-fluorescein isothiocyanate (FITC) were obtained from PharMingen. Hexadimethrine bromide (Polybrene) was purchased from Sigma and DOTAP was purchased from Boehringer-Mannheim. WebImmunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21242-1-AP (E2A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Jurkat cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. simulating bouncing with different gravity

Mouse TCF-3/E2A Antibody MAB7650: R&D Systems

WebFeb 24, 2024 · E2A Antibody (Yae) is an IgG1 κ mouse monoclonal E2A antibody (also designated E2A antibody or TCF3 antibody) suitable for the detection of ITF-1, and E2A isoforms E12 and E47 of mouse, rat and human origin by WB, IP and IF. E2A Antibody (Yae) is available as the non-conjugated anti-E2A antibody form, as well as multiple … WebE2A Antibodies. Santa Cruz Biotechnology, Inc. offers a broad range of E2A antibodies. Select E2A antibodies from monoclonal antibodies listed below. View detailed E2A antibody specifications by linking to the specific product blocks. Select appropriate E2A antibodies for your research by isotype, epitope, applications and species reactivity. Webwith antibodies to the Myc-tag (9E10), E12/47 (E2A.E12), or phos-pho E2A pT355 (Bethyl Laboratories) as indicated. Generation of Stable Cell Lines Expressing MyoD-ER and North-ern Blot Analysis The MDER-SN construct containing both the chimeric MyoD-ER cDNA and neo selectable marker gene (Hollenberg et al., 1993) was rcvs board

E2A CRISPR Knockout and Activation Products (h) - SCBT

E2A Polyclonal Antibody (21242-1-AP) - Thermo Fisher …

WebAll lanes : Anti-TCF3 / E2A antibody (ab69999) at 1/500 dilution Lane 1 : Extracts from 293 cells, minus immunising peptide Lane 2 : Extracts from 293 cells, plus immunising … WebE2A Antibody. 4865 is a primary antibody from Cell Signaling Technology. 7 Citations Host: Rabbit Clonality: Polyclonal Validations: None Available Applications: WB. Reactivity: Homo sapiens (Human) Mouse anti Human Vitamin D Receptor:Preservative Free. MCA3543Z is a primary antibody from Bio-Rad (Formerly AbD Serotec) rcvs approved practicesWebAntibodies that detect TCF3 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Flow Cytometry. These antibodies target TCF3 in Human, Mouse, Rat and Non-human primate samples. Our TCF3 polyclonal, monoclonal and recombinant monoclonal antibodies are … rcvs charity

"WebThe translocation results in the transcription of a unique chimeric protein E2A-Pbx1 with an amino acid sequences not present on E2A or Pbx alone. E2A-Pbx1 is found only in cells with the t (1,19) translocation. It is detectable in the nucleus of 697 cell line (a pre-B cell line). E2A-Pbx1 is considered to be an oncoprotein, however, the ... " - E2a antibody

E2a antibody

WebJan 25, 2015 · Linking the heavy chain (HC) and light chain (LC) genes required for monoclonal antibodies (mAb) production on a single cassette using 2A peptides allows control of LC and HC ratio and reduces non-expressing cells. Four 2A peptides derived from the foot-and-mouth disease virus (F2A), equine rhinitis A virus (E2A), porcine … WebE2A Antibody (V-18) is a rabbit polyclonal IgG; 200 µg/ml. epitope mapping at the C-terminus of E2A of human origin. Discontinued polyclonal antibody. See product citations (38)

Did you know?

WebDuring the transition of CLP into B-cell lineages via pre–pro-B cells, transcription factors E2A, early B cell factor 1 (EBF1), and PAX5 play crucial combined roles in a hierarchical …

WebMay 3, 2024 · E2A Antibody (G-2) is a mouse monoclonal IgG 1 κ E2A antibody, cited in 7 publications, provided at 200 µg/ml. E2A Antibody (G-2) is recommended for detection … WebGeneral description. Anti-E2A Antibody detects endogenous levels of total E2A protein. The E2A gene is mapped to human chromosome 19p13.3. [ 1] The protein encoded is a …

WebAll lanes : Anti-TCF3 / E2A antibody [7H3] (ab243152) at 0.537 µg/ml Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate … WebB-lymphocytes can modify their immunoglobulin (Ig) genes to generate specific antibodies with a new isotype and enhanced affinity against an antigen. Activation-induced cytidine deaminase (AID), which is positively regulated by the transcription factor E2A, is the key enzyme that initiates these processes by deaminating cytosine to uracil in Ig ...

WebThere are no reviews for TCF-3/E2A Antibody (NBP2-67437). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen; Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen; Submit a Review. Product General Protocols ...

WebApr 8, 2024 · I ChIP assays were performed in A549 cells using a specific E2A antibody (anti-E47) or a control rabbit normal IgG (anti-IgG) and primers specific for P1, P2, and P3. A randomly segment (−40 to ... rcvs charterWebexpression in an E2A / pre-pro-B cell line, in pro-B cells isolated from Rag1-orMb1-deficient mice, and in a panel of Abelson transformed pro-B-cell lines (Rag-deficient ... combination, and antibody production by immature/transitional 1 B cells. J Immunol 178: 3593–3601. von Boehmer H, Melchers F. 2010. Checkpoints in lymphocyte develop- simulating gravity in spaceWebTCF-3/E2A was detected in immersion fixed frozen sections of E9.5 mouse embryo using Rat Anti-Mouse TCF-3/E2A Monoclonal Antibody (Catalog # MAB7650) at 10 µg/mL overnight at 4 °C. Tissue was stained using the … rcvs change of addressWebGeneral notes. ab251582 is the carrier-free version of ab224638. Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency. This conjugation-ready format is designed for use with ... simulating grazing beef and sheep systemsWebThe guide RNA in the E2A Lentiviral Activation Particles (h2) encodes a target-specific 20 nt guide RNA that differs from the guide RNA in E2A Lentiviral Activation Particles (h) Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: E2A Antibody (G-2): sc-133075 rcvs code of conduct diagnosisWebGeneral description. Anti-E2A Antibody detects endogenous levels of total E2A protein. The E2A gene is mapped to human chromosome 19p13.3. [ 1] The protein encoded is a T-cell factor/lymphoid enhancer factor transcription factor and … rcvs code of conduct clinical recordsWebTCF3 (E2A) is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell … rcvs breach of code of conduct